Nanotechnology in Phytotherapy: The Effect of Noni’s Nanoemulsion on Bacterial Translocation Induced By Experimental Model of Intestinal Ischemia and Reperfusion by Irami Araújo-Filho in The Research and Reviews on Healthcare: Open Access Journal in Lupine Publishers
Background:
The study evaluated nano structured extract
Morinda citrifolia L. (Noni) conveyed in enteral form, in an experimental model
of intestinal ischemia/reperfusion (I/R), as well as in the prevention of bacterial translocation.
Purpose:
Observe the protective and repairing effect of Noni’s nanoemulsified extract in the presence of induced bacterial translocation, by an experimental model of intestinal ischemia/reperfusion.
Methods and Findings:
The study consisted of 2 groups of 7 animals, where the Controls were treated with 0.9% saline solution (I/R + S) and Experimental group (I/R + Noni) treated with 5 mg/ml/Kg Noni nanoemulsion, orally by gavage 12h and 2h before the experiment. In the groups, the superior mesenteric artery was occluded with a vascular micro clamp and the laparotomy reopened 60min. after for pull back the clamp. Treatment response was assessed by blood count, inflammatory markers and biochemical dosages, including a sample of the terminal ileum and hepatic of each animal was harvested, fixed in formalin 10% and included in paraffin and stained with hematoxylin-eosin for morphometric measurement. Measurement of TNF-α, IL-1β, IL-6, and IL-10 was done. One gram of spleen, liver, and mesenteric lymph nodes were harvested for culture by selective means for Gram (-) and Gram (+) bacteria. ANOVA and the post-hoc Turkey and Student ́s t test were used, considering p<0, 05 as significant.
Conclusion:
Noni ́s nanoemulsion positively influenced the organic reactions in the presence of intestinal ischemia/reperfusion, reducing the production of pro inflammatory cytokines, bacterial translocation, preventing tissue injury and attenuating the systemic inflammatory response against the experimental model used.
The study evaluated nano structured extract
Morinda citrifolia L. (Noni) conveyed in enteral form, in an experimental model
of intestinal ischemia/reperfusion (I/R), as well as in the prevention of bacterial translocation.
Purpose:
Observe the protective and repairing effect of Noni’s nanoemulsified extract in the presence of induced bacterial translocation, by an experimental model of intestinal ischemia/reperfusion.
Methods and Findings:
The study consisted of 2 groups of 7 animals, where the Controls were treated with 0.9% saline solution (I/R + S) and Experimental group (I/R + Noni) treated with 5 mg/ml/Kg Noni nanoemulsion, orally by gavage 12h and 2h before the experiment. In the groups, the superior mesenteric artery was occluded with a vascular micro clamp and the laparotomy reopened 60min. after for pull back the clamp. Treatment response was assessed by blood count, inflammatory markers and biochemical dosages, including a sample of the terminal ileum and hepatic of each animal was harvested, fixed in formalin 10% and included in paraffin and stained with hematoxylin-eosin for morphometric measurement. Measurement of TNF-α, IL-1β, IL-6, and IL-10 was done. One gram of spleen, liver, and mesenteric lymph nodes were harvested for culture by selective means for Gram (-) and Gram (+) bacteria. ANOVA and the post-hoc Turkey and Student ́s t test were used, considering p<0, 05 as significant.
Conclusion:
Noni ́s nanoemulsion positively influenced the organic reactions in the presence of intestinal ischemia/reperfusion, reducing the production of pro inflammatory cytokines, bacterial translocation, preventing tissue injury and attenuating the systemic inflammatory response against the experimental model used.
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